Transcriptome analysis in primary neural stem cells using a tag cDNA amplification method.

ABSTRACT:

Background

Neural stem cells (NSCs) can be isolated from the adult mammalian brain and expanded in culture, in the form of cellular aggregates called neurospheres. Neurospheres provide an in vitro model for studying NSC behaviour and give information on the factors and mechanisms that govern their proliferation and differentiation. They are also a promising source for cell replacement therapies of the central nervous system. Neurospheres are complex structures consisting of several cell types of varying degrees of differentiation. One way of characterising neurospheres is to analyse their gene expression profiles. The value of such studies is however uncertain since they are heterogeneous structures and different populations of neurospheres may vary significantly in their gene expr

SUBMITTER: Sievertzon M 

PROVIDER: S-EPMC1112599 | biostudies-literature | 2005 Apr

REPOSITORIES: biostudies-literature