Molecular Surveillance, Prevalence, and Distribution of Cacao Infecting Badnavirus Species in Cote d'Ivoire and Ghana.

ABSTRACT: The cacao swollen shoot disease (CSSD) caused by a complex of badnavirus species presents a major challenge for cacao production in West Africa, especially Ghana and Côte d'Ivoire. In this study, CSSD species detection efficiency, diversity, and geographic distribution patterns in cacao plantations in Ghana and Côte d'Ivoire were investigated through field surveillance, PCR detection assays, sequencing of positive amplicons, and phylogeographic clustering. Cumulatively, the detection efficiency of the tested CSSD primer sets that were targeting the movement protein domain of the virus ranged from 0.15% (CSSD-3 primer) to 66.91% (CSSD-1 primer) on all the symptomatic cacao leaf samples assessed. The identified CSSD species differed phylogenetically and overlapped in distribution, with the c

SUBMITTER: Ameyaw GA 

PROVIDER: S-EPMC11126031 | biostudies-literature | 2024 May

REPOSITORIES: biostudies-literature