Severe neurological impairment and immune function: altered neutrophils, monocytes, T lymphocytes, and inflammasome activation.

ABSTRACT:

Background

Infections cause significant morbidity and mortality in children with Severe Neurological Impairment (SNI). Alterations in immune cell numbers and function in children with neurodisability have been reported. We aimed to characterise neutrophil, monocyte and lymphocyte proportions and activation, at baseline and in response to stimulation with lipopolysaccharide, in children with SNI compared to healthy controls.

Methods

Whole blood samples of children with SNI and controls were incubated in the presence or absence of lipopolysaccharide (10 ng/ml). Monocyte and neutrophil function (Cluster of Differentiation (CD)11b, (TLR)-4 and CD66b expression) and lymphocytes were assessed by flow cytometry. Expression of genes involved in the inflammasome (NLR Family Pyrin Dom

SUBMITTER: Allen J 

PROVIDER: S-EPMC11126379 | biostudies-literature | 2024 May

REPOSITORIES: biostudies-literature