Discovery, characterization, and engineering of an advantageous Streptomyces host for heterologous expression of natural product biosynthetic gene clusters.

ABSTRACT:

Background

Streptomyces is renowned for its robust biosynthetic capacity in producing medically relevant natural products. However, the majority of natural products biosynthetic gene clusters (BGCs) either yield low amounts of natural products or remain cryptic under standard laboratory conditions. Various heterologous production hosts have been engineered to address these challenges, and yet the successful activation of BGCs has still been limited. In our search for a valuable addition to the heterologous host panel, we identified the strain Streptomyces sp. A4420, which exhibited rapid initial growth and a high metabolic capacity, prompting further exploration of its potential.

Results

We engineered a polyketide-focused chassis strain based on Streptomyces sp. A4420 (CH st

SUBMITTER: Klumbys E 

PROVIDER: S-EPMC11127301 | biostudies-literature | 2024 May

REPOSITORIES: biostudies-literature