Global soil metagenomics reveals distribution and predominance of Deltaproteobacteria in nitrogen-fixing microbiome.

ABSTRACT:

Background

Biological nitrogen fixation is a fundamental process sustaining all life on earth. While distribution and diversity of N₂-fixing soil microbes have been investigated by numerous PCR amplicon sequencing of nitrogenase genes, their comprehensive understanding has been hindered by lack of de facto standard protocols for amplicon surveys and possible PCR biases. Here, by fully leveraging the planetary collections of soil shotgun metagenomes along with recently expanded culture collections, we evaluated the global distribution and diversity of terrestrial diazotrophic microbiome.

Results

After the extensive analysis of 1,451 soil metagenomic samples, we revealed that the Anaeromyxobacteraceae and Geobacteraceae within Deltaproteobacteria are ubiquitous grou

SUBMITTER: Masuda Y 

PROVIDER: S-EPMC11127431 | biostudies-literature | 2024 May

REPOSITORIES: biostudies-literature