Evaluation of fecal sample collection methods for feline gut microbiome profiling: fecal loop vs. litter box.

ABSTRACT:

Introduction

Microbial population structures within fecal samples are vital for disease screening, diagnosis, and gut microbiome research. The two primary methods for collecting feline fecal samples are: (1) using a fecal loop, which retrieves a rectal sample using a small, looped instrument, and (2) using the litter box, which collects stool directly from the litter. Each method has its own advantages and disadvantages and is suitable for different research objectives.

Methods and results

Whole-genome shotgun metagenomic sequencing were performed on the gut microbiomes of fecal samples collected using these two methods from 10 adult cats housed in the same research facility. We evaluated the influence of collection methods on feline microbiome analysis, particularly their i

SUBMITTER: Ma X 

PROVIDER: S-EPMC11127567 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature