Inhibition of Phlpp1 preserves the mechanical integrity of articular cartilage in a murine model of post-traumatic osteoarthritis.

ABSTRACT:

Objective

Phlpp1 inhibition is a potential therapeutic strategy for cartilage regeneration and prevention of post-traumatic osteoarthritis (PTOA). To understand how Phlpp1 loss affects cartilage structure, cartilage elastic modulus was measured with atomic force microscopy (AFM) in male and female mice after injury.

Methods

Osteoarthritis was induced in male and female Wildtype (WT) and Phlpp1^-/- mice by destabilization of the medial meniscus (DMM). At various timepoints post-injury, activity was measured, and knee joints examined with AFM and histology. In another cohort of WT mice, the PHLPP inhibitor NSC117079 was intra-articularly injected 4 weeks after injury.

Results

Male WT mice showed decreased activity and histological signs of cartilage damage at

SUBMITTER: Arnold KM 

PROVIDER: S-EPMC11127785 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature