Cloning and characterization of a novel mesophilic xylanase gene <i>Fgxyn3</i> from <i>Fusarium graminearum</i> Z-1.

ABSTRACT: In order to search for high specific activity and the resistant xylanases to XIP-I and provide more alternative xylanases for industrial production, a strain of Fusarium graminearum from Triticum aestivum grains infected with filamentous fungus produced xylanases was isolated and identified. Three xylanase genes from Fusarium graminearum Z-1 were cloned and successfully expressed in E. coli and P. pastoris, respectively. The specific activities of Fgxyn1, _EFgxyn2 and _EFgxyn3 for birchwood xylan were 38.79, 0.85 and 243.83 U/mg in E. coli, and 40.11, 0 and 910.37 U/mg in P. pastoris, respectively. _EFgxyn3 and _PFgxyn3 had the similar optimum pH at 6.0 and pH

SUBMITTER: Zhang D 

PROVIDER: S-EPMC11127905 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature