Multicentre analysis of nucleic acid quantification using aqueous humour liquid biopsy in uveal melanoma: implications for clinical testing.

ABSTRACT:

Objective

Uveal melanoma (UM) tumour biopsy is limited by size and intratumour heterogeneity. We explored the potential of aqueous humour (AH) liquid biopsy for UM by quantifying analytes in samples collected at diagnosis and after brachytherapy to look for clinical correlations with tumour features.

Design

Case-series study.

Participants

Sixty-six UM patients and 16 control subjects from a tertiary care hospital.

Methods

The study included 119 UM AH samples and 16 control samples analyzed for unprocessed analytes (i.e., dsDNA, miRNA, and protein) using Qubit fluorescence assays.

Results

Analytes were widely quantifiable among available UM AH samples (dsDNA: 94.1%; miRNA: 88.0%; protein: 95.2%) at significantly higher concentrations than among control sa

SUBMITTER: Pike SB 

PROVIDER: S-EPMC11128479 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature