Deciphering the Diversity in Bacterial Transporters That Salvage Queuosine Precursors.

ABSTRACT: Queuosine (Q) is a modification of the wobble base of tRNA harboring GUN anticodons with roles in decoding accuracy and efficiency. Its synthesis is complex with multiple enzymatic steps, and several pathway intermediates can be salvaged. The only two transporter families known to salvage Q precursors are QPTR/COG1738 and QrtT/QueT. Analyses of the distribution of known Q synthesis and salvage genes in human gut and oral microbiota genomes have suggested that more transporter families remain to be found and that Q precursor exchanges must occur within the structured microenvironments of the mammalian host. Using physical clustering and fusion-based association with Q salvage genes, candidate genes for missing transporters were identified and five were tested experimentally by complementati

SUBMITTER: Quaiyum S 

PROVIDER: S-EPMC11130926 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature