Transient theory for scanning electrochemical microscopy of biological membrane transport: uncovering membrane-permeant interactions.

ABSTRACT: Scanning electrochemical microscopy (SECM) has emerged as a powerful method to quantitatively investigate the transport of molecules and ions across various biological membranes as represented by living cells. Advantageously, SECM allows for the in situ and non-destructive imaging and measurement of high membrane permeability under simple steady-state conditions, thereby facilitating quantitative data analysis. The SECM method, however, has not provided any information about the interactions of a transported species, i.e., a permeant, with a membrane through its components, e.g., lipids, channels, and carriers. Herein, we propose theoretically that SECM enables the quantitative investigation of membrane-permeant interactions by employing transient conditions. Specifica

SUBMITTER: Huang SH 

PROVIDER: S-EPMC11131039 | biostudies-literature | 2024 May

REPOSITORIES: biostudies-literature