Low-frequency HIV-1 drug resistance mutations in antiretroviral naive individuals in Botswana.

ABSTRACT:

Background

Individuals living with human immunodeficiency virus (HIV) who experience virological failure (VF) after combination antiretroviral therapy (cART) initiation may have had low-frequency drug resistance mutations (DRMs) at cART initiation. There are no data on low-frequency DRMs among cART-naïve HIV-positive individuals in Botswana.

Methods

We evaluated the prevalence of low-frequency DRMs among cART-naïve individuals previously sequenced using Sanger sequencing. The generated pol amplicons were sequenced by next-generation sequencing.

Results

We observed low-frequency DRMs (detected at <20% in 33/103 (32%) of the successfully sequenced individuals, of whom four also had mutations detected at >20%. K65R was the most common low-frequency DRM detected in 8 indi

SUBMITTER: Maruapula D 

PROVIDER: S-EPMC11132386 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature