CHOGlycoNET: Comprehensive glycosylation reaction network for CHO cells.

ABSTRACT: Chinese hamster ovary (CHO) cells are extensively used for the production of glycoprotein therapeutics proteins, for which N-linked glycans are a critical quality attribute due to their influence on activity and immunogenicity. Manipulation of protein glycosylation is commonly achieved through cell or process engineering, which are often guided by mathematical models. However, each study considers a unique glycosylation reaction network that is tailored around the cell line and product at hand. Herein, we use 200 glycan datasets for both recombinantly produced and native proteins from different CHO cell lines to reconstruct a comprehensive reaction network, CHOGlycoNET, based on the individual minimal reaction networks describing each dataset. CHOGlycoNET is used to investigate the distrib

SUBMITTER: Kotidis P 

PROVIDER: S-EPMC11132536 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature