Effect of nonsense-mediated mRNA decay factor SMG9 deficiency on premature aging in zebrafish.

ABSTRACT: SMG9 is an essential component of the nonsense-mediated mRNA decay (NMD) machinery, a quality control mechanism that selectively degrades aberrant transcripts. Mutations in SMG9 are associated with heart and brain malformation syndrome (HBMS). However, the molecular mechanism underlying HBMS remains unclear. We generated smg9 mutant zebrafish (smg9^oi7/oi7) that have a lifespan of approximately 6 months or longer, allowing for analysis of the in vivo function of Smg9 in adults in more detail. smg9^oi7/oi7 zebrafish display congenital brain abnormalities and reduced cardiac contraction. Additionally, smg9^oi7/oi7 zebrafish exhibit a premature aging phenotype. Analysis of NMD target mRNAs shows a trend toward increased mRNA levels in smg9^oi7/oi7 zebra

SUBMITTER: Lai S 

PROVIDER: S-EPMC11133409 | biostudies-literature | 2024 May

REPOSITORIES: biostudies-literature