Discovery of a lagriamide polyketide by integrated genome mining, isotopic labeling, and untargeted metabolomics.

ABSTRACT: Microorganisms from the order Burkholderiales have been the source of a number of important classes of natural products in recent years. For example, study of the beetle-associated symbiont Burkholderia gladioli led to the discovery of the antifungal polyketide lagriamide; an important molecule from the perspectives of both biotechnology and chemical ecology. As part of a wider project to sequence Burkholderiales genomes from our in-house Burkholderiales library we identified a strain containing a biosynthetic gene cluster (BGC) similar to the original lagriamide BGC. Structure prediction failed to identify any candidate masses for the products of this BGC from untargeted metabolomics mass spectrometry data. However, genome mining from publicly available databases identified fragmen

SUBMITTER: Fergusson CH 

PROVIDER: S-EPMC11134395 | biostudies-literature | 2024 May

REPOSITORIES: biostudies-literature