RNase III processing of intervening sequences found in helix 9 of 23S rRNA in the alpha subclass of Proteobacteria.

ABSTRACT: We provide experimental evidence for RNase III-dependent processing in helix 9 of the 23S rRNA as a general feature of many species in the alpha subclass of Proteobacteria (alpha-Proteobacteria). We investigated 12 Rhodobacter, Rhizobium, Sinorhizobium, Rhodopseudomonas, and Bartonella strains. The processed region is characterized by the presence of intervening sequences (IVSs). The 23S rDNA sequences between positions 109 and 205 (Escherichia coli numbering) were determined, and potential secondary structures are proposed. Comparison of the IVSs indicates very different evolutionary rates in some phylogenetic branches, lateral genetic transfer, and evolution by insertion and/or deletion. We show that the IVS processing in Rhodobacter capsulatus in vivo is RNase III-dependent and that RNa

SUBMITTER: Evguenieva-Hackenberg E 

PROVIDER: S-EPMC111346 | biostudies-literature | 2000 Sep

REPOSITORIES: biostudies-literature