<i>Circ-0000953</i> deficiency exacerbates podocyte injury and autophagy disorder by targeting <i>Mir665-3p-Atg4b</i> in diabetic nephropathy.

ABSTRACT: Circular RNAs (circRNAs) are special non-coding RNA (ncRNA) molecules that play a significant role in many diseases. However, the biogenesis and regulation of circRNAs in diabetic nephropathy (DN) are largely unknown. Here, we investigated the expression profile of circRNAs in kidney of DN mice through circular RNA sequencing (circRNA-seq). The renal biopsy samples of patients with DN had low circ -0,000,953 expression, which was significantly associated with renal function. Furthermore, loss-of-function and gain-of-function experiments were carried out to prove the role of circ -0,000,953 in DN. Podocyte conditional knockin (cKI) or systemic overexpression of circ -0,000,953 alleviated albuminuria and restored macroautophagy/autophagy in kidney of diabetic mice. Howev

SUBMITTER: Liu X 

PROVIDER: S-EPMC11135827 | biostudies-literature | 2024 May

REPOSITORIES: biostudies-literature