MitoBK_Ca is functionally expressed in murine and human breast cancer cells and potentially contributes to metabolic reprogramming.

ABSTRACT: Alterations in the function of K⁺ channels such as the voltage- and Ca²⁺-activated K⁺ channel of large conductance (BK_Ca) reportedly promote breast cancer (BC) development and progression. Underlying molecular mechanisms remain, however, elusive. Here, we provide electrophysiological evidence for a BK_Ca splice variant localized to the inner mitochondrial membrane of murine and human BC cells (mitoBK_Ca). Through a combination of genetic knockdown and knockout along with a cell permeable BK_Ca channel blocker, we show that mitoBK_Ca modulates overall cellular and mitochondrial energy production, and mediates the metabolic rewiring referred to as the 'Warburg effect', thereby promoting BC cell proliferation

SUBMITTER: Bischof H 

PROVIDER: S-EPMC11136494 | biostudies-literature | 2024 May

REPOSITORIES: biostudies-literature