Relationship between circadian rhythm-related genes and extracellular matrix: implications for sleep deprivation.

ABSTRACT:

Background

Sleep deprivation (SD) can lead to the development of various pathological disorders. The extracellular matrix (ECM) compositions and circadian rhythm genes are two pivotal variables of SD. However, their relationships remain undefined during SD.

Methods

A mouse SD model was established using a modified multiplatform water environment method. The expression of nerve growth factor (NGF) in mouse hippocampus was detected by an immunofluorescence (IF) method. Protein expression was assessed by western blot, and mRNA analysis was performed by quantitative real-time PCR (qRT-PCR). The differentially expressed genes after SD, the genes associated with stromal score, and gene expression correlation were analyzed by bioinformatic analysis.

Results

The mouse model o

SUBMITTER: Liu X 

PROVIDER: S-EPMC11136824 | biostudies-literature | 2024 May

REPOSITORIES: biostudies-literature