COPD basal cells are primed towards secretory to multiciliated cell imbalance driving increased resilience to environmental stressors.

ABSTRACT:

Introduction

Environmental pollutants injure the mucociliary elevator, thereby provoking disease progression in chronic obstructive pulmonary disease (COPD). Epithelial resilience mechanisms to environmental nanoparticles in health and disease are poorly characterised.

Methods

We delineated the impact of prevalent pollutants such as carbon and zinc oxide nanoparticles, on cellular function and progeny in primary human bronchial epithelial cells (pHBECs) from end-stage COPD (COPD-IV, n=4), early disease (COPD-II, n=3) and pulmonary healthy individuals (n=4). After nanoparticle exposure of pHBECs at air-liquid interface, cell cultures were characterised by functional assays, transcriptome and protein analysis, complemented by single-cell analysis in serial samples of pHBEC cul

SUBMITTER: Stoleriu MG 

PROVIDER: S-EPMC11137452 | biostudies-literature | 2024 May

REPOSITORIES: biostudies-literature