Quantification of ssDNA Scaffold Production by Ion-Pair Reverse Phase Chromatography.

ABSTRACT: DNA origami is an emerging technology that can be used as a nanoscale platform in numerous applications ranging from drug delivery systems to biosensors. The DNA nanostructures are assembled from large single-stranded DNA (ssDNA) scaffolds, ranging from hundreds to thousands of nucleotides and from short staple strands. Scaffolds are usually obtained by asymmetric PCR (aPCR) or Escherichia coli infection/transformation with phages or phagemids. Scaffold quantification is typically based on agarose gel electrophoresis densitometry for molecules obtained by aPCR, or by UV absorbance, in the case of scaffolds obtained by infection or transformation. Although these methods are well-established and easy-to-apply, the results obtained are often inaccurate due to the lack of selectivity an

SUBMITTER: Silva-Santos AR 

PROVIDER: S-EPMC11137683 | biostudies-literature | 2024 May

REPOSITORIES: biostudies-literature