Distinct properties of putative trophoblast stem cells established from somatic cell nuclear-transferred pig blastocysts.

ABSTRACT:

Background

Genetically modified pigs are considered ideal models for studying human diseases and potential sources for xenotransplantation research. However, the somatic cell nuclear transfer (SCNT) technique utilized to generate these cloned pig models has low efficiency, and fetal development is limited due to placental abnormalities.

Results

In this study, we unprecedentedly established putative porcine trophoblast stem cells (TSCs) using SCNT and in vitro-fertilized (IVF) blastocysts through the activation of Wing-less/Integrated (Wnt) and epidermal growth factor (EGF) pathways, inhibition of transforming growth factor-β (TGFβ) and Rho-associated protein kinase (ROCK) pathways, and supplementation with ascorbic acid. We also compared the transcripts of putative TSCs orig

SUBMITTER: Kim E 

PROVIDER: S-EPMC11137969 | biostudies-literature | 2024 May

REPOSITORIES: biostudies-literature