PCP auto count: a novel Fiji/ImageJ plug-in for automated quantification of planar cell polarity and cell counting.

ABSTRACT: Introdution: During development, planes of cells give rise to complex tissues and organs. The proper functioning of these tissues is critically dependent on proper inter- and intra-cellular spatial orientation, a feature known as planar cell polarity (PCP). To study the genetic and environmental factors affecting planar cell polarity, investigators must often manually measure cell orientations, which is a time-consuming endeavor. To automate cell counting and planar cell polarity data collection we developed a Fiji/ImageJ plug-in called PCP Auto Count (PCPA). Methods: PCPA analyzes binary images and identifies "chunks" of white pixels that contain "caves" of infiltrated black pixels. For validation, inner ear sensory epithelia including cochleae and utricles from mice were im

SUBMITTER: Stansak KL 

PROVIDER: S-EPMC11140036 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature