Expression of different-size transcripts from the clpP-clpX operon of Escherichia coli during carbon deprivation.

ABSTRACT: Transcription of the clpP-clpX operon of Escherichia coli leads to the production of two different sizes of transcripts. In log phase, the level of the longer transcript is higher than the level of the shorter transcript. Soon after the onset of carbon starvation, the level of the shorter transcript increases significantly, and the level of the longer transcript decreases. The longer transcript consists of the entire clpP-clpX operon, whereas the shorter transcript contains the entire clpP gene but none of the clpX coding sequence. The RpoH protein is required for the increase in the level of the shorter transcript during carbon starvation. Primer extension experiments suggest that there is increased usage of the sigma(32)-dependent promoter of the clpP-clpX operon within 15 min after the

SUBMITTER: Li C 

PROVIDER: S-EPMC111403 | biostudies-literature | 2000 Dec

REPOSITORIES: biostudies-literature