Impaired dynamic X-chromosome inactivation maintenance in T cells is a feature of spontaneous murine SLE that is exacerbated in female-biased models.

ABSTRACT:

Objective

Systemic lupus erythematosus (SLE) is a highly female-biased systemic autoimmune disease, but the molecular basis for this female bias remains incompletely elucidated. B and T lymphocytes from patients with SLE and female-biased mouse models of SLE exhibit features of epigenetic dysregulation on the X chromosome which may contribute to this strong female bias. We therefore examined the fidelity of dynamic X-chromosome inactivation maintenance (dXCIm) in the pathogenesis of two murine models of spontaneous lupus-NZM2328 and MRL/lpr-with disparate levels of female-bias to determine whether impaired dXCIm contributes to the female bias of disease.

Methods

CD23⁺ B cells and CD3⁺ T cells were purified from age-matched C57BL/6 (B6), MRL/lpr, and NZM

SUBMITTER: Jiwrajka N 

PROVIDER: S-EPMC11140471 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature