TomoNet: A streamlined cryogenic electron tomography software pipeline with automatic particle picking on flexible lattices.

ABSTRACT: Cryogenic electron tomography (cryoET) is capable of determining in situ biological structures of molecular complexes at near-atomic resolution by averaging half a million subtomograms. While abundant complexes/particles are often clustered in arrays, precisely locating and seamlessly averaging such particles across many tomograms present major challenges. Here, we developed TomoNet, a software package with a modern graphical user interface to carry out the entire pipeline of cryoET and subtomogram averaging to achieve high resolution. TomoNet features built-in automatic particle picking and three-dimensional (3D) classification functions and integrates commonly used packages to streamline high-resolution subtomogram averaging for structures in 1D, 2D, or 3D arrays. Automatic partic

SUBMITTER: Wang H 

PROVIDER: S-EPMC11140495 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature