Fmp40 ampylase regulates cell survival upon oxidative stress by controlling Prx1 and Trx3 oxidation.

ABSTRACT: Reactive oxygen species (ROS), play important roles in cellular signaling, nonetheless are toxic at higher concentrations. Cells have many interconnected, overlapped or backup systems to neutralize ROS, but their regulatory mechanisms remain poorly understood. Here, we reveal an essential role for mitochondrial AMPylase Fmp40 from budding yeast in regulating the redox states of the mitochondrial 1-Cys peroxiredoxin Prx1, which is the only protein shown to neutralize H₂O₂ with the oxidation of the mitochondrial glutathione and the thioredoxin Trx3, directly involved in the reduction of Prx1. Deletion of FMP40 impacts a cellular response to H₂O₂ treatment that leads to programmed cell death (PCD) induction and an adaptive response involving up or d

SUBMITTER: Masanta S 

PROVIDER: S-EPMC11140801 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature