Ampicillin detection using absorbance biosensors utilizing Mn-doped ZnS capped with chitosan micromaterials.

ABSTRACT: The detection of ampicillin plays a crucial role in managing and monitoring its usage and resistance. This study introduces a simple and effective biosensor for ampicillin detection, utilizing the unique absorbance features of Mn-doped ZnS capped by chitosan micromaterials in conjunction with β-lactamase activity. The biosensors can detect ampicillin concentrations from 13.1 to 72.2 μM, with a minimum detection limit of 2.93 μM for sensors based on 300 mg/L of the sensing material. In addition, these sensors show high specificity for ampicillin over other antibiotics such as penicillin, tetracycline, amoxicillin, cephalexin, and a non-antibiotic-glucose. This specificity is demonstrated by an enhancing effect when beta-lactamase is used, as opposed to a quenching effect observed at 340 nm

SUBMITTER: Nguyen SH 

PROVIDER: S-EPMC11141450 | biostudies-literature | 2024 May

REPOSITORIES: biostudies-literature