Deep Transcriptomics Reveals Cell-Specific Isoforms of Pan-Neuronal Genes.

ABSTRACT: Profiling gene expression in single neurons using single-cell RNA-Seq is a powerful method for understanding the molecular diversity of the nervous system. Profiling alternative splicing in single neurons using these methods is more challenging, however, due to low capture efficiency and sensitivity. As a result, we know much less about splicing patterns and regulation across neurons than we do about gene expression. Here we leverage unique attributes of the C. elegans nervous system to investigate deep cell-specific transcriptomes complete with biological replicates generated by the CeNGEN consortium, enabling high-confidence assessment of splicing across neuron types even for lowly-expressed genes. Global splicing maps reveal several striking observations, including pan-neuronal g

SUBMITTER: Wolfe Z 

PROVIDER: S-EPMC11142100 | biostudies-literature | 2024 May

REPOSITORIES: biostudies-literature