Structural Determinants of Vibrio cholerae FeoB Nucleotide Promiscuity.

ABSTRACT: Ferrous iron (Fe²⁺) is required for the growth and virulence of many pathogenic bacteria, including Vibrio cholerae (Vc), the causative agent of the disease cholera. For this bacterium, Feo is the primary system that transports Fe²⁺ into the cytosol. FeoB, the main component of this system, is regulated by a soluble cytosolic domain termed NFeoB. Recent reanalysis has shown that NFeoBs can be classified as either GTP-specific or NTP-promiscuous, but the structural and mechanistic bases for these differences were not known. To explore this intriguing property of FeoB, we solved the X-ray crystal structures of VcNFeoB in both the apo and GDP-bound forms. Surprisingly, this promiscuous NTPase displayed a canonical NFeoB G-protein fold like GTP-specif

SUBMITTER: Lee M 

PROVIDER: S-EPMC11142208 | biostudies-literature | 2024 May

REPOSITORIES: biostudies-literature