Single Cell Profiling Distinguishes Leukemia-Selective Chemotypes.

ABSTRACT: A central challenge in chemical biology is to distinguish molecular families in which small structural changes trigger large changes in cell biology. Such families might be ideal scaffolds for developing cell-selective chemical effectors - for example, molecules that activate DNA damage responses in malignant cells while sparing healthy cells. Across closely related structural variants, subtle structural changes have the potential to result in contrasting bioactivity patterns across different cell types. Here, we tested a 600-compound Diversity Set of screening molecules from the Boston University Center for Molecular Discovery (BU-CMD) in a novel phospho-flow assay that tracked fundamental cell biological processes, including DNA damage response, apoptosis, M-phase cell cycle, and protein

SUBMITTER: Thirman HL 

PROVIDER: S-EPMC11142275 | biostudies-literature | 2024 May

REPOSITORIES: biostudies-literature