Rational engineering of Halomonas salifodinae to enhance hydroxyectoine production under lower-salt conditions.

ABSTRACT: Hydroxyectoine is an important compatible solute that holds potential for development into a high-value chemical with broad applications. However, the traditional high-salt fermentation for hydroxyectoine production presents challenges in treating the high-salt wastewater. Here, we report the rational engineering of Halomonas salifodinae to improve the bioproduction of hydroxyectoine under lower-salt conditions. The comparative transcriptomic analysis suggested that the increased expression of ectD gene encoding ectoine hydroxylase (EctD) and the decreased expressions of genes responsible for tricarboxylic acid (TCA) cycle contributed to the increased hydroxyectoine production in H. salifodinae IM328 grown under high-salt conditions. By blocking the degradation pathway of ectoine and hydro

SUBMITTER: Yang N 

PROVIDER: S-EPMC11142988 | biostudies-literature | 2024 May

REPOSITORIES: biostudies-literature