Expression and purification of the mitochondrial transmembrane protein FAM210A in Escherichia coli.

ABSTRACT: The protein Family with sequence similarity 210 member A (FAM210A) is a mitochondrial inner membrane protein that regulates the protein synthesis of mitochondrial DNA encoded genes. However, how it functions in this process is not well understood. Developing and optimizing a protein purification strategy will facilitate biochemical and structural studies of FAM210A. Here, we developed a method to purify human FAM210A with deleted mitochondrial targeting signal sequence using the MBP-His₁₀ fusion in Escherichia coli. The recombinant FAM210A protein was inserted into the E. coli cell membrane and purified from isolated bacterial cell membranes, followed by a two-step process using Ni-NTA resin-based immobilized-metal affinity chromatography (IMAC) and ion exchange purification. A

SUBMITTER: Hollinger J 

PROVIDER: S-EPMC11143474 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature