Engineering cascade biocatalysis in whole cells for syringic acid bioproduction.

ABSTRACT:

Background

Syringic acid (SA) is a high-value natural compound with diverse biological activities and wide applications, commonly found in fruits, vegetables, and herbs. SA is primarily produced through chemical synthesis, nonetheless, these chemical methods have many drawbacks, such as considerable equipment requirements, harsh reaction conditions, expensive catalysts, and numerous by-products. Therefore, in this study, a novel biotransformation route for SA production was designed and developed by using engineered whole cells.

Results

An O-methyltransferase from Desulfuromonas acetoxidans (DesAOMT), which preferentially catalyzes a methyl transfer reaction on the meta-hydroxyl group of catechol analogues, was identified. The whole cells expressing DesAOMT can transform gal

SUBMITTER: Liu X 

PROVIDER: S-EPMC11143566 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature