X-chromosome inactivation in human iPSCs provides insight into X-regulated gene expression in autosomes.

ABSTRACT:

Background

Variation in X chromosome inactivation (XCI) in human-induced pluripotent stem cells (hiPSCs) can impact their ability to model biological sex biases. The gene-wise landscape of X chromosome gene dosage remains unresolved in female hiPSCs. To characterize patterns of de-repression and escape from inactivation, we performed a systematic survey of allele specific expression in 165 female hiPSC lines.

Results

XCI erosion is non-random and primarily affects genes that escape XCI in human tissues. Individual genes and cell lines vary in the frequency and degree of de-repression. Bi-allelic expression increases gradually after modest decrease of XIST in cultures, whose loss is commonly used to mark lines with eroded XCI. We identify three clusters of female lines at dif

SUBMITTER: Topa H 

PROVIDER: S-EPMC11143737 | biostudies-literature | 2024 May

REPOSITORIES: biostudies-literature