Hsa-miR-548v controls the viscoelastic properties of human cardiomyocytes and improves their relaxation rates.

ABSTRACT: The impairment of left ventricular (LV) diastolic function with an inadequate increase in myocardial relaxation velocity directly results in lower LV compliance, increased LV filling pressures, and heart failure symptoms. The development of agents facilitating the relaxation of human cardiomyocytes requires a better understanding of the underlying regulatory mechanisms. We performed a high-content microscopy-based screening in human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) using a library of 2,565 human miRNA mimics and measured relaxation kinetics via high-computing analyses of motion movies. We identified hsa-miR-548v, a primate-specific miRNA, as the miRNA producing the largest increase in relaxation velocities. This positive lusitropic effect was reproduced in e

SUBMITTER: Vermersch E 

PROVIDER: S-EPMC11143964 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature