Survival of transfused red blood cells from a donor with alpha-thalassemia trait in a recipient with sickle cell disease.

ABSTRACT:

Background

Post-transfusion survival of donor red blood cells (RBCs) is important for effective chronic transfusion therapy in conditions including sickle cell disease (SCD). Biotin labeling RBCs allows direct in vivo measurement of multiple donor RBC units simultaneously post-transfusion.

Study design and methods

In an observational trial of patients with SCD receiving monthly chronic transfusion therapy, aliquots of RBCs from one transfusion episode were biotin-labeled and infused along with the unlabeled RBC units. Serial blood samples were obtained to measure RBC survival. Donor units were tested for RBC indices, hemoglobin fractionation, and glucose-6-phosphate dehydrogenase (G6PD) enzyme activity. For microcytic donor RBCs (MCV < 70 fL), HBA1 and HBA2 genetic testing w

SUBMITTER: Yee MEM 

PROVIDER: S-EPMC11144116 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature