TALEN-mediated homologous-recombination-based fibroin light chain in-fusion expression system in Bombyx mori.

ABSTRACT: Silkworm was the first domesticated insect and has important economic value. It has also become an ideal model organism with applications in genetic and expression studies. In recent years, the use of transgenic strategies has made the silkworm silk gland an attractive bioreactor for the production of recombinant proteins, in particular, piggyBac-mediated transgenes. However, owing to differences in regulatory elements such as promoters, the expression levels of exogenous proteins have not reached expectations. Here, we used targeted gene editing to achieve site-specific integration of exogenous genes on genomic DNA and established the fibroin light chain (FibL) in-fusion expression system by TALEN-mediated homology-directed recombination. First, the histidine-rich cuticular protein

SUBMITTER: Yu S 

PROVIDER: S-EPMC11144906 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature