Two widely spaced initiator binding sites create an HMG1-dependent parvovirus rolling-hairpin replication origin.

ABSTRACT: Minute virus of mice (MVM) replicates via a linearized form of rolling-circle replication in which the viral nickase, NS1, initiates DNA synthesis by introducing a site-specific nick into either of two distinct origin sequences. In vitro nicking and replication assays with substrates that had deletions or mutations were used to explore the sequences and structural elements essential for activity of one of these origins, located in the right-end (5') viral telomere. This structure contains 248 nucleotides, most-favorably arranged as a simple hairpin with six unpaired bases. However, a pair of opposing NS1 binding sites, located near its outboard end, create a 33-bp palindrome that could potentially assume an alternate cruciform configuration and hence directly bind HMG1, the essential cofac

SUBMITTER: Cotmore SF 

PROVIDER: S-EPMC111468 | biostudies-literature | 2000 Feb

REPOSITORIES: biostudies-literature