The importance of DNA sequence for nucleosome positioning in transcriptional regulation.

ABSTRACT: Nucleosome positioning is a key factor for transcriptional regulation. Nucleosomes regulate the dynamic accessibility of chromatin and interact with the transcription machinery at every stage. Influences to steer nucleosome positioning are diverse, and the according importance of the DNA sequence in contrast to active chromatin remodeling has been the subject of long discussion. In this study, we evaluate the functional role of DNA sequence for all major elements along the process of transcription. We developed a random forest classifier based on local DNA structure that assesses the sequence-intrinsic support for nucleosome positioning. On this basis, we created a simple data resource that we applied genome-wide to the human genome. In our comprehensive analysis, we found a special role o

SUBMITTER: Sahrhage M 

PROVIDER: S-EPMC11147951 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature