Substrate scope expansion of 4-phenol oxidases by rational enzyme selection and sequence-function relations.

ABSTRACT: Enzymes are natures' catalysts and will have a lasting impact on (organic) synthesis as they possess unchallenged regio- and stereo selectivity. On the downside, this high selectivity limits enzymes' substrate range and hampers their universal application. Therefore, substrate scope expansion of enzyme families by either modification of known biocatalysts or identification of new members is a key challenge in enzyme-driven catalysis. Here, we present a streamlined approach to rationally select enzymes with proposed functionalities from the ever-increasing amount of available sequence data. In a case study on 4-phenol oxidoreductases, eight enzymes of the oxidase branch were selected from 292 sequences on basis of the properties of first shell residues of the catalytic pocket, guided by the

SUBMITTER: Eggerichs D 

PROVIDER: S-EPMC11148156 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature