Sonogenetics-controlled synthetic designer cells for cancer therapy in tumor mouse models.

ABSTRACT: Bacteria-based therapies are powerful strategies for cancer therapy, yet their clinical application is limited by a lack of tunable genetic switches to safely regulate the local expression and release of therapeutic cargoes. Rapid advances in remote-control technologies have enabled precise control of biological processes in time and space. We developed therapeutically active engineered bacteria mediated by a sono-activatable integrated gene circuit based on the thermosensitive transcriptional repressor TlpA₃₉. Through promoter engineering and ribosome binding site screening, we achieved ultrasound (US)-induced protein expression and secretion in engineered bacteria with minimal noise and high induction efficiency. Specifically, delivered either intratumorally or intravenously,

SUBMITTER: Gao T 

PROVIDER: S-EPMC11148564 | biostudies-literature | 2024 May

REPOSITORIES: biostudies-literature