The Transpeptidase Sortase A Binds Nucleic Acids and Mediates Mammalian Cell Labeling.

ABSTRACT: Wild-type sortase A is an important virulence factor displaying a diverse array of proteins on the surface of bacteria. This protein display relies on the transpeptidase activity of sortase A, which is widely engineered to allow protein ligation and protein engineering based on the interaction between sortase A and peptides. Here an unknown interaction is found between sortase A from Staphylococcus aureus and nucleic acids, in which exogenously expressed engineered sortase A binds oligonucleotides in vitro and is independent of its canonical transpeptidase activity. When incubated with mammalian cells, engineered sortase A further mediates oligonucleotide labeling to the cell surface, where sortase A attaches itself and is part of the labeled moiety. The labeling reaction can also be media

SUBMITTER: Liu Y 

PROVIDER: S-EPMC11151058 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature