Whole-genome assembly of a hybrid Trypanosoma cruzi strain assembled with Nanopore sequencing alone.

ABSTRACT: Trypanosoma cruzi is the causative agent of Chagas disease, which causes 10,000 deaths per year. Despite the high mortality associated with Chagas, relatively few parasite genomes have been assembled to date, with genome assemblies unavailable even for some commonly used laboratory strains. This is at least partially due to T. cruzi's highly complex and highly repetitive genome, which defies investigation using traditional short-read sequencing methods. In this study, we have generated a high-quality whole-genome assembly of the hybrid Tulahuen strain, a commercially available type VI strain, using long-read Nanopore sequencing without short-read scaffolding. The assembled genome contains 25% repeat regions, 17% variable multigene family members, and 27% transposable elements (TEs) and is

SUBMITTER: Hakim JMC 

PROVIDER: S-EPMC11152063 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature