Transcription decouples estrogen-dependent changes in enhancer-promoter contact frequencies and spatial proximity.

ABSTRACT: How enhancers regulate their target genes in the context of 3D chromatin organization is extensively studied and models which do not require direct enhancer-promoter contact have recently emerged. Here, we use the activation of estrogen receptor-dependent enhancers in a breast cancer cell line to study enhancer-promoter communication at two loci. This allows high temporal resolution tracking of molecular events from hormone stimulation to efficient gene activation. We examine how both enhancer-promoter spatial proximity assayed by DNA fluorescence in situ hybridization, and contact frequencies resulting from chromatin in situ fragmentation and proximity ligation, change dynamically during enhancer-driven gene activation. These orthogonal methods produce seemingly paradoxical results: upon

SUBMITTER: Gomez Acuna LI 

PROVIDER: S-EPMC11152312 | biostudies-literature | 2024 May

REPOSITORIES: biostudies-literature