Unveiling the dynamics of acetylation and phosphorylation in SGBS and 3T3-L1 adipogenesis.

ABSTRACT: Obesity, characterized by enlarged and dysfunctional adipose tissue, is among today's most pressing global public health challenges with continuously increasing prevalence. Despite the importance of post-translational protein modifications (PTMs) in cellular signaling, knowledge of their impact on adipogenesis remains limited. Here, we studied the temporal dynamics of transcriptome, proteome, central carbon metabolites, and the acetyl- and phosphoproteome during adipogenesis using LC-MS/MS combined with PTM enrichment strategies on human (SGBS) and mouse (3T3-L1) adipocyte models. Both cell lines exhibited unique PTM profiles during adipogenesis, with acetylated proteins being enriched for central energy metabolism, while phosphorylated proteins related to insulin signaling and organizatio

SUBMITTER: Aldehoff AS 

PROVIDER: S-EPMC11152682 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature