Molecular basis for differential Igk versus Igh V(D)J joining mechanisms.

ABSTRACT: In developing B cells, V(D)J recombination assembles exons encoding IgH and Igκ variable regions from hundreds of gene segments clustered across Igh and Igk loci. V, D and J gene segments are flanked by conserved recombination signal sequences (RSSs) that target RAG endonuclease¹. RAG orchestrates Igh V(D)J recombination upon capturing a J_H-RSS within the J_H-RSS-based recombination centre^1-3 (RC). J_H-RSS orientation programmes RAG to scan upstream D- and V_H-containing chromatin that is presented in a linear manner by cohesin-mediated loop extrusion^4-7. During Igh scanning, RAG robustly utilizes only D-RSSs or V_H-RSSs in convergent (deletional) orientation with J_H-RSSs^4-7. However,

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC11153149 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature