Quantitative detection of chikungunya, Zika, and dengue viruses by one-step real-time PCR in different cell substrates.

ABSTRACT: Chikungunya (CHIKV), Zika (ZIKV), and dengue viruses (DENV) are vector-borne pathogens that cause emerging and re-emerging epidemics throughout tropical and subtropical countries. The symptomatology is similar among these viruses and frequently co-circulates in the same areas, making the diagnosis arduous. Although there are different methods for detecting and quantifying pathogens, real-time reverse transcription-polymerase chain reaction (real-time RT-qPCR) has become a leading technique for detecting viruses. However, the currently developed assays frequently involve probes and high-cost reagents, limiting access in low-income countries. Therefore, this study aims to design and evaluate a quantitative one-step RT-qPCR assay to detect CHIKV, ZIKV, and DENV with high specificity, reproduc

SUBMITTER: Cuellar-Quimbaya AF 

PROVIDER: S-EPMC11153482 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature