TGF-β signalling limits effector function capacity of NK cell anti-tumour immunity in human bladder cancer.

ABSTRACT:

Background

Natural killer (NK) cells are important innate immunity players and have unique abilities to recognize and eliminate cancer cells, particularly in settings of antibody-opsonization and antibody-dependant cellular cytotoxicity (ADCC). However, NK cell-based responses in bladder cancers to therapeutic antibodies are typically immunosuppressed, and these immunosuppressive mechanisms are largely unknown.

Methods

Single cell RNA sequencing (scRNA-seq) and high-dimensional flow cytometry were used to investigate the phenotype of tumour-infiltrating NK cells in patients with bladder cancer. Further, in vitro, and in vivo models of this disease were used to validate these findings.

Findings

NK cells within bladder tumours displayed reduced expression of FcγRIIIa/CD

SUBMITTER: Wong JKM 

PROVIDER: S-EPMC11154198 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature