Growth behavior and mRNA expression profiling during growth of IPEC-J2 cells.

ABSTRACT:

Objective

The IPEC-J2 cell line is used as an in vitro small intestine model for swine, but it is also used as a model for the human intestine, presenting a relatively unique setting. By combining electric cell-substrate impedance sensing, with next-generation-sequencing technology, we showed that mRNA gene expression profiles and related pathways can depend on the growth phase of IPEC-J2 cells. Our investigative approach welcomes scientists to reproduce or modify our protocols and endorses putting their gene expression data in the context of the respective growth phase of the cells.

Results

Three time points are presented: (TP1) 1 h after medium change (= 6 h after seeding of cells), (TP2) the time point of the first derivative maximum of the cell growth curve, and a third

SUBMITTER: Binder ARD 

PROVIDER: S-EPMC11155027 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature